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Nanjing Jiancheng Bioengineering Research Institute Co Ltd triglycerides
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Triglyceride, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applygen Technologies tissue triglyceride content assay kit
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Tissue Triglyceride Content Assay Kit, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd triglyceride assay kit
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Triglyceride Assay Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applygen Technologies tissue triglyceride tg content assay kit
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Tissue Triglyceride Tg Content Assay Kit, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fisher Scientific triglyceride standard
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
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Applygen Technologies intracellular triglyceride
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd triglyceride content
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the <t>triglyceride</t> content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Journal: iScience

Article Title: Integration of GWAS and eQTL analysis reveals regulatory CNVs for fatty acid traits in cattle

doi: 10.1016/j.isci.2026.116180

Figure Lengend Snippet: Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Article Snippet: Cellular triglyceride (TG) content was measured using Applygen's Tissue Triglyceride Content Assay Kit (E1013), and protein concentration was determined using a BCA Protein Quantification Kit (P1511).

Techniques: Functional Assay, Biomarker Discovery, Expressing, Transfection, Comparison, Knockdown, Control, Negative Control

Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Journal: iScience

Article Title: Integration of GWAS and eQTL analysis reveals regulatory CNVs for fatty acid traits in cattle

doi: 10.1016/j.isci.2026.116180

Figure Lengend Snippet: Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Article Snippet: Tissue Triglyceride (TG) Content Assay Kit , Applygen , https://applygen.com/?m=home&c=View&a=index&aid=1151.

Techniques: Functional Assay, Biomarker Discovery, Expressing, Transfection, Comparison, Knockdown, Control, Negative Control